COMT ELISA Kit (Rat) (OKCD02342)

Catalog No: OKCD02342

Size:96WELLS

Rat COMT ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	93-101%	91-104%	85-92%	79-96%
  EDTA Plasma (n=5)	79-101%	90-99%	80-99%	92-101%
  Heparin Plasma (n=5)	79-104%	99-105%	90-105%	85-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Catechol-O-Methyltransferase (COMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Catechol-O-Methyltransferase (COMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Catechol-O-Methyltransferase (COMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Catechol-O-Methyltransferase (COMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	95-103	98
  EDTA Plasma (n=5)	89-104	94
  Heparin Plasma(n=5)	94-101	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Catechol-O-Methyltransferase (COMT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Catechol-O-Methyltransferase (COMT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-COMT Microplate: 96 Wells (12 x 8 Well strips)
  • COMT Lyophilized Standard: 2
  • 100X Biotinylated COMT Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.67 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Catechol-O-Methyltransferase (COMT). No significant cross-reactivity or interference between Catechol-O-Methyltransferase (COMT) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Comt
• Gene Full Name: catechol-O-methyltransferase
• Alias Symbols: catechol O-methyltransferase;catecholamine-O-methyltransferase.
• NCBI Gene Id: 24267
• Protein Name: Catechol O-methyltransferase
• Description of Target: Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
• Uniprot ID: P22734
• Protein Accession #: NP_036663.1
• Nucleotide Accession #: NM_012531.2
• Protein Size (# AA): 264