- Kit Component:
Aviva has included in this kit the best reagents capable of active or passive blocking, which can be used together or individually to eliminate assay inference:
TRU BlockTM Catalog # OAMA04082; 1 vial = 50mg (~25mg/mL, 2mL volume)
TRU BlockTM2 Catalog # OOMA00069; 1 vial = 50mg (~25mg/mL, 2mL volume)
TRU BlockTM3 Catalog # OOMA00070; 1 vial = 50mg (~25mg/mL, 2mL volume)
Mouse IgG, 9‐13mg/mL Catalog # OAMA04134; 1 vial = 20mg (9‐13mg/mL , ~2mL volume)
Mouse IgG, 50-55mg/mL Catalog #OAMA04133; 1 vial = 20mg (50-55mg/mL , ~2mL volume)
IgM Assay Diluent Catalog #OOMA00071; 2 vials = 10mL each
- Printable datasheet/reference manual for Complete Blocker Kit for Immunoassay Development(OKMA00001) OKMA00001
- Aviva Systems Biology Complete Blocker Kit features the best proven reagents designed to prevent false positive and false negative results and enhance assay performance. Immunoassay interference is typically caused by non‐specific interactions between antibodies within the assay itself and endogenous antibodies present within a patient’s sample. Any type of interference can cause an assay to report a false result. A blocking reagent is a product designed to prevent interfering antibodies from adversely affecting immunoassay results.
There are two main types of blocking reagents: passive and active. A passive blocker is a preparation of normal animal immunoglobulins usually added in excess concentration to prevent interfering antibodies from binding to the assay antibody components by providing alternate/competitive binding sites. An active blocker is designed to remove all types of heterophilic interference. It contains a specific binder directed against human heterophilic antibodies and once bound to the interfering antibodies, block further binding to other assay components through steric hindrance. Active blockers can typically be used in lower concentrations than passive blocking reagents.
TRU Block is an active blocker that is designed to stop HAMA and other heterophilic interference that can cause false positive results. TRU Block 2 and TRU Block 3 are variations of the original TRU BlockTM formulation with the same fundamental blocking characteristics. All formulations contain normal mouse serum and proprietary ingredients that neutralize human heterophilic antibodies. TRU Block can be added directly to assay buffers, conjugates or immobilized on lateral flow membranes. It is suitable for sandwich and competitive immunoassays and effective at low concentrations (e.g. 5ug/mL). Assay data demonstrates superior performance in blocking HAMA and RF interference compared to competitors.
- Additional Information:
- PROTOCOL FOR MOUSE IGG
Mouse IgG, 9-13mg/mL OAMA04134 Mouse IgG, 50-55mg/mL OAMA04133
STORAGE CONDITIONS: Short‐term: 2-8C. Long term: ‐20C. Avoid multiple freeze and thaw cycles.
PRESERVATIVE: 0.05% Sodium azide
1. For optimum performance, Mouse IgG should be in contact with the patient samples prior to contact with monoclonal antibodies in the assay. Anti‐mouse antibodies in the patient sample will bind to purified Mouse IgG and will be blocked from binding to the monoclonal antibodies used in the assay.
2. Use Mouse IgG by placing it in the Sample Diluent, the Conjugate Diluent, or drying it down as a blocking stripe ahead of the test stripe in a Lateral flow assay.
3. Mouse IgG should be added at 10x the concentration of the monoclonal antibodies being used in the assay (example: if using 5ug/mL of MAb/conjugate, add 50ug/mL Mouse IgG). This makes the patient samples' anti-mouse antibodies 10x more likely to bind to the mouse IgG and not interfere with the assay MAbs.
4. Each assay format is different; the optimal working concentration and location of the blocker in the assay must be determined for each specific application.
PROTOCOL FOR IGM ASSAY DILUENT
IgM Assay Diluent OOMA00071
STORAGE CONDITIONS: Short‐term: 2-8C.
PRESERVATIVE: 0.1% Sodium azide
1. IgM Assay Diluent should be stored at 2‐8C. Diluent must be warmed to room temperature prior to use.
2. Dilute the patient serum sample in the IgM Assay diluent at a 1:21 dilution or greater. The diluent must be standardized as a unit with the other assay components.
3. It is recommended that the dilutions are performed in a separate tube. Mix well after diluting.
- Mix-n-StainTM R-PE Antibody Compatibility and Labeling Protocol Selection Guide
Component Compatibility Sodium Azide Compatible, proceed to Section B Glycerol Perform ultrafiltration (Section A) Tris Compatible, proceed to Section B Glycine Compatible, proceed to Section B BSA or gelatin Up to 4X IgG (ug amount): Compatible, proceed to Section B
More than 4X IgG (ug amount): Not compatible, purify IgG
Ascites fluid Not compatible, purify IgG Serum Not compatible, purify IgG Hybridoma supernatant Not compatible: purify IgG
- Tips Information: