COLEC11 Antibody - N-terminal region (OAAB03942)

Catalog No: OAAB03942

Size:400ul

Western blot analysis of COLEC11 Antibody (N-term) in MDA-MB231 cell lysates (35ug/lane). COLEC11 (arrow) was detected using the purified Pab.

Western blot analysis of COLEC11 (arrow) using rabbit polyclonal COLEC11 Antibody (N-term) either nontransfected (Lane 1) or lysates transiently transfected transfected (Lane 2) with the COLEC11 gene.

Formalin-fixed and paraffin-embedded human hepatocarcinoma with COLEC11 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of MDA-231 cells using COLEC11 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Additional Information: Function: Lectin that binds to various sugars including fucose and mannose. Has a higher affinity for fucose compared to mannose. Does not bind to glucose, N-acetylglucosamine and N- acetylgalactosamine. Also binds lipopolysaccharides (LPS). Involved in fundamental development serving as a guidance cue for neural crest cell migration (By similarity).
  Cellular Location: Secreted.
  Tissue Location: Ubiquitous.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This COLEC11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-78 amino acids from the N-terminal region of human COLEC11.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Intended Use: COLEC11 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: Flow cytometric analysis of MDA-231 cells using COLEC11 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: COLEC11
• Alias Symbols: 3MC2, CLK1, CL-K1-I, CL-K1-II, CL-K1-IIa, CL-K1-IIb
• NCBI Gene Id: 78989
• Protein Name: Collectin-11
• Uniprot ID: Q9BWP8
• Protein Accession #: NP_001242911.1
• Molecular Weight: 28665 Da