COL6A1 ELISA Kit (Human) (OKCD01638)

Catalog No: OKCD01638 (Formerly GWB-KBBVQ9)

Size:96WELLS

Human COL6A1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	84-93%	81-99%	86-101%	96-105%
  EDTA Plasma (n=5)	85-95%	95-103%	88-96%	89-96%
  Heparin Plasma (n=5)	80-96%	91-99%	92-101%	79-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type VI Alpha 1 (COL6a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type VI Alpha 1 (COL6a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type VI Alpha 1 (COL6a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Collagen Type VI Alpha 1 (COL6a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 62.5-4000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	88-95	92
  EDTA Plasma (n=5)	81-92	87
  Heparin Plasma (n=5)	87-97	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type VI Alpha 1 (COL6a1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type VI Alpha 1 (COL6a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-COL6A1 Microplate: 96 Wells (12 x 8 Well Strips)
  • COL6A1 Lyophilized Standard: 2 x 4 ng
  • 100X Biotinylated COL6A1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 23.5 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type VI Alpha 1 (COL6a1). No significant cross-reactivity or interference between Collagen Type VI Alpha 1 (COL6a1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: COL6A1
• Gene Full Name: collagen type VI alpha 1 chain
• Alias Symbols: alpha 1 (VI) chain (61 AA);BTHLM1;collagen alpha-1(VI) chain;collagen VI, alpha-1 polypeptide;collagen, type VI, alpha 1;epididymis secretory sperm binding protein;OPLL;UCHMD1.
• NCBI Gene Id: 1291
• Protein Name: Collagen alpha-1(VI) chain
• Description of Target: Collagen VI acts as a cell-binding protein.
• Uniprot ID: P12109
• Protein Accession #: NP_001839.2
• Nucleotide Accession #: NM_001848.2
• Protein Size (# AA): 1028