Catalog No: OKEH02328
Size:96 Wells
Price: $625.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology COL4A3BP ELISA Kit (Human) (OKEH02328) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for COL4A3BP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for COL4A3BP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample COL4A3BP captured in well.
ELISA Kit Range0.156 - 10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.4% (n = 20)
Mean Inter-assay CV%: < 9.7% (n = 20)
ELISA Kit Component
COL4A3BP Microplate96 Wells (12 x 8 Well strips)
COL4A3BP Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated COL4A3BP Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Collagen type IV alpha-3-binding protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCOL4A3BP
Gene Full Namecollagen type IV alpha 3 binding protein
NCBI Gene Id10087
Protein NameCollagen type IV alpha-3-binding protein
Description of TargetThis gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene.
Swissprot IdQ9Y5P4
Protein Accession #NP_001123577.1
Nucleotide Accession #NM_001130105.1
Protein Size (# AA)624
Molecular Weight71 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express COL4A3BP.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express COL4A3BP.
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