COL3A1 ELISA Kit (Mouse) (OKCD00190)

Catalog No: OKCD00190 (Formerly GWB-KBBOW2)

Size:96WELLS

Mouse Col3a1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	82-96%	95-104%	96-103%	83-98%
  EDTA plasma(n=5)	81-93%	86-101%	87-95%	78-93%
  heparin plasma(n=5)	94-102%	82-94%	93-102%	80-96%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type III Alpha 1 (COL3a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type III Alpha 1 (COL3a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type III Alpha 1 (COL3a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Collagen Type III Alpha 1 (COL3a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 4.7-300ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	78-105	101
  EDTA plasma(n=5)	94-102	99
  heparin plasma(n=5)	86-102	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type III Alpha 1 (COL3a1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type III Alpha 1 (COL3a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Col3a1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Col3a1 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated Col3a1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 6.4 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type III Alpha 1 (COL3a1). No significant cross-reactivity or interference between Collagen Type III Alpha 1 (COL3a1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Col1a1
• Gene Full Name: collagen, type I, alpha 1
• Alias Symbols: alpha-1 type 1 collagen;alpha-1 type I collagen;Co;Col;Col1;Col1a-1;Cola1;Cola-1;collagen alpha-1(I) chain;Mov-;Mov13;Mov-13;procollagen, type I, alpha 1.
• NCBI Gene Id: 12842
• Protein Name: Collagen alpha-1(I) chain
• Description of Target: Type I collagen is a member of group I collagen (fibrillar forming collagen).
• Uniprot ID: P11087
• Protein Accession #: NP_034060
• Nucleotide Accession #: NM_009930.2
• Protein Size (# AA): 1464