COL2A1 ELISA Kit (Human) (OKCD06183)

Catalog No: OKCD06183

Size:96 Wells

COL2A1 ELISA Kit (Human) (OKCD06183) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen II N-Terminal Propeptide (PIINP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Procollagen II N-Terminal Propeptide (PIINP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen II N-Terminal Propeptide (PIINP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Procollagen II N-Terminal Propeptide (PIINP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 93.75-6,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen II N-Terminal Propeptide (PIINP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen II N-Terminal Propeptide (PIINP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • COL2A1 Microplate: 96 Wells (12 x 8 Well strips)
  • COL2A1 Lyophilized Standard: 2
  • 100X COL2A1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 37.55pg/mL

Target Info

• Gene Symbol: COL2A1
• Gene Full Name: collagen type II alpha 1 chain
• Alias Symbols: alpha-1 type II collagen;ANFH;AOM;arthroophthalmopathy, progressive (Stickler syndrome);cartilage collagen;chondrocalcin;COL11A3;collagen alpha-1(II) chain;collagen II, alpha-1 polypeptide;collagen, type II, alpha 1;SEDC;STL1.
• NCBI Gene Id: 1280
• Protein Name: Collagen alpha-1(II) chain
• Description of Target: Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
• Uniprot ID: P02458
• Protein Accession #: NP_001835.3
• Nucleotide Accession #: NM_001844.4