Catalog No: OKEH04140
Size:96T
Price: $525.00
SKU
OKEH04140
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology COL28A1 ELISA Kit (Human) (OKEH04140) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for COL28A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for COL28A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample COL28A1 captured in the well.
ELISA Kit Range0.156-10nmol/L
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.2% (n = 20)
Mean Inter-assay CV%: <= 10.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-COL28A1 Microplate96 Wells (12 x 8 Well Strips)
COL28A1 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
COL28A1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.032nmol/L
SpecificityNatural and recombinant Human Collagen alpha-1(XXVIII) chain
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCOL28A1
Gene Full Namecollagen type XXVIII alpha 1 chain
Alias SymbolsCOL28;collagen alpha-1(XXVIII) chain;collagen, type XXVIII, alpha 1.
NCBI Gene Id340267
Protein NameQ2UY09
Description of TargetCollagen alpha-1(XXVIII) chain
Uniprot IDhttps://www.uniprot.org/uniprot/Q2UY09
Protein Accession #NP_001032852.2
Nucleotide Accession #NM_001037763.2
Protein Size (# AA)1125
Molecular Weight117 kDa
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