COL1a2 ELISA kit (Human) (OKCD00157)

Catalog No: OKCD00157 (Formerly GWB-KBBBS8)

Size:96WELLS

Human COL1A2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: ELISA-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	89-96%	84-101%	80-92%	78-94%
  EDTA plasma(n=5)	88-102%	81-103%	85-101%	85-94%
  heparin plasma(n=5)	79-97%	79-92%	81-103%	94-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I Alpha 2 (COL1a2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type I Alpha 2 (COL1a2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type I Alpha 2 (COL1a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Collagen Type I Alpha 2 (COL1a2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	81-91	85
  EDTA plasma(n=5)	82-95	90
  heparin plasma(n=5)	91-101	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type I Alpha 2 (COL1a2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type I Alpha 2 (COL1a2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-COL1A2 Microplate: 96 Wells (12 x 8 Well Strips)
  • COL1A2 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated COL1A2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 6.3 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type I Alpha 2 (COL1a2). No significant cross-reactivity or interference between Collagen Type I Alpha 2 (COL1a2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: COL1A2
• Gene Full Name: collagen type I alpha 2 chain
• Alias Symbols: alpha 2 type I procollagen;alpha 2(I) procollagen;alpha 2(I)-collagen;alpha-2 type I collagen;collagen alpha-2(I) chain;collagen I, alpha-2 polypeptide;collagen of skin, tendon and bone, alpha-2 chain;collagen, type I, alpha 2;EDSARTH2;EDSCV;epididymis secretory sperm binding protein;OI4;type I procollagen.
• NCBI Gene Id: 1278
• Protein Name: Collagen alpha-2(I) chain
• Description of Target: Type I collagen is a member of group I collagen (fibrillar forming collagen).
• Uniprot ID: P08123
• Protein Accession #: NP_000080
• Nucleotide Accession #: NM_000089.3
• Protein Size (# AA): 1366