Size:96 Wells
$569.00
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SKU
OKCD00157
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COL1A2 ELISA Kit (Human) (OKCD00157)

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Catalog#: OKCD00157
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
This product is Genway GWB-KBBBS8
More Information
Predicted Species Reactivity Human
Application ELISA
Kit Detection Method Colorimetric, OD 450 nm
Kit Duration 2.5 Hours
Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)89-96%84-101%80-92%78-94%
EDTA plasma(n=5)88-102%81-103%85-101%85-94%
heparin plasma(n=5)79-97%79-92%81-103%94-101%
Kit Principle Aviva Systems Biology COL1A2 ELISA Kit (Human) (OKCD00157) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for COL1A2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for COL1A2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample COL1A2captured in well.
Kit Range 15.62-1,000 pg/mL
Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)81-9185
EDTA plasma(n=5)82-9590
heparin plasma(n=5)91-10197
Kit Reproducibility Intra-assay Precision: 3 samples with known low, middle and high level COL1A2 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level COL1A2 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Component
ComponentAmount
Anti-COL1A2 Microplate96 Wells (12 x 8 Well Strips)
COL1A2 Lyophilized Standard2 x 1 ng
100X Biotinylated COL1A2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, plasma, tissue homogenates and other biological fluids
Sensitivity < 6.3 pg/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay has high sensitivity and excellent specificity for detection of Collagen Type I Alpha 2 (COL1a2). No significant cross-reactivity or interference between Collagen Type I Alpha 2 (COL1a2) and analogues was observed.
Assay Info Assay Methodology: Quantitative Sandwich Immunoassay
Gene Symbol COL1A2
Official Gene Full Name collagen type I alpha 2 chain
Alias Symbols Alpha-2 type I collagen, Collagen alpha-2(I) chain
NCBI Gene Id 1278
Protein Name collagen alpha-2(I) chain
Description of Target Type I collagen is a member of group I collagen (fibrillar forming collagen).
Swissprot Id P08123
Protein Accession # NP_000080
Nucleotide Accession # NM_000089.3
Protein Size (# AA) 1366
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express COL1A2.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express COL1A2.
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