Catalog No: OKCD00157 (Formerly GWB-KBBBS8)
Size:96 Wells
Price: $650.00
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COL1A2 ELISA Kit (Human) (OKCD00157)

100% of 100
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
EDTA plasma(n=5)88-102%81-103%85-101%85-94%
heparin plasma(n=5)79-97%79-92%81-103%94-101%
ELISA Kit PrincipleAviva Systems Biology COL1A2 ELISA Kit (Human) (OKCD00157) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for COL1A2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for COL1A2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample COL1A2captured in well.
ELISA Kit Range15.62-1,000 pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
EDTA plasma(n=5)82-9590
heparin plasma(n=5)91-10197
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level COL1A2 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level COL1A2 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
Anti-COL1A2 Microplate96 Wells (12 x 8 Well Strips)
COL1A2 Lyophilized Standard2 x 1 ng
100X Biotinylated COL1A2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 6.3 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Collagen Type I Alpha 2 (COL1a2). No significant cross-reactivity or interference between Collagen Type I Alpha 2 (COL1a2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCOL1A2
Gene Full Namecollagen type I alpha 2 chain
Alias SymbolsOI4, EDSCV, EDSARTH2
NCBI Gene Id1278
Protein Namecollagen alpha-2(I) chain
Description of TargetType I collagen is a member of group I collagen (fibrillar forming collagen).
Swissprot IdP08123
Protein Accession #NP_000080
Nucleotide Accession #NM_000089.3
Protein Size (# AA)1366
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express COL1A2.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express COL1A2.
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