Catalog No: OKCD00157 (Formerly GWB-KBBBS8)
Price: $650.00
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COL1a2 ELISA kit (Human) (OKCD00157)

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Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
EDTA plasma(n=5)88-102%81-103%85-101%85-94%
heparin plasma(n=5)79-97%79-92%81-103%94-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I Alpha 2 (COL1a2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type I Alpha 2 (COL1a2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type I Alpha 2 (COL1a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Collagen Type I Alpha 2 (COL1a2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
EDTA plasma(n=5)82-9590
heparin plasma(n=5)91-10197
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type I Alpha 2 (COL1a2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type I Alpha 2 (COL1a2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
Anti-COL1A2 Microplate96 Wells (12 x 8 Well Strips)
COL1A2 Lyophilized Standard2 x 1 ng
100X Biotinylated COL1A2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 6.3 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Collagen Type I Alpha 2 (COL1a2). No significant cross-reactivity or interference between Collagen Type I Alpha 2 (COL1a2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCOL1A2
Gene Full Namecollagen type I alpha 2 chain
Alias Symbolsalpha 2 type I procollagen;alpha 2(I) procollagen;alpha 2(I)-collagen;alpha-2 type I collagen;collagen alpha-2(I) chain;collagen I, alpha-2 polypeptide;collagen of skin, tendon and bone, alpha-2 chain;collagen, type I, alpha 2;EDSARTH2;EDSCV;epididymis secretory sperm binding protein;OI4;type I procollagen.
NCBI Gene Id1278
Protein NameP08123
Description of TargetCollagen alpha-2(I) chain
Uniprot ID
Protein Accession #NP_000080
Nucleotide Accession #NM_000089.3
Protein Size (# AA)1366
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