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COL1A2 ELISA Kit (Human) (OKCD00157)

96 Wells
$569.00
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Application:
ELISA
Gene Symbol:
COL1A2
Official Gene Full Name:
collagen type I alpha 2 chain
NCBI Gene Id:
1278
Alias Symbols:
Alpha-2 type I collagen, Collagen alpha-2(I) chain
Description of Target:
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Protein Name:
collagen alpha-2(I) chain
Swissprot Id:
P08123
Protein Accession #:
NP_000080
Nucleotide Accession #:
NM_000089.3
Predicted Species Reactivity:
Human
Sample Type:
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
< 6.3 pg/mL
Kit Range:
15.62-1,000 pg/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level COL1A2 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level COL1A2 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology COL1A2 ELISA Kit (Human) (OKCD00157) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for COL1A2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for COL1A2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample COL1A2captured in well.
Kit Component:
ComponentAmount
Anti-COL1A2 Microplate96 Wells (12 x 8 Well Strips)
COL1A2 Lyophilized Standard2 x 1 ng
100X Biotinylated COL1A2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Linearity:
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)89-96%84-101%80-92%78-94%
EDTA plasma(n=5)88-102%81-103%85-101%85-94%
heparin plasma(n=5)79-97%79-92%81-103%94-101%
Kit Recovery:
MatrixRecovery Range (%)Average (%)
serum(n=5)81-9185
EDTA plasma(n=5)82-9590
heparin plasma(n=5)91-10197
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express COL1A2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express COL1A2.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Collagen Type I Alpha 2 (COL1a2). No significant cross-reactivity or interference between Collagen Type I Alpha 2 (COL1a2) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protein Size (# AA):
1366

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78/03/2019 03:01
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Fibroblasts in ELISA

Submitted by:
Ernst Reichenberger, PhD
UConn Health (UCH)

COL1A2

1. What sample types were used with this kit?

We used cell culture supernatants from fibroblasts treated with and without TGF-b1.

2. Were these samples validated or characterized as positive and/or negative controls?

Samples were validated as functional fibroblasts.

3. What dilution worked for your samples?

We didn’t dilute the cell culture supernatant.

4. Please provide a brief summary of your sample preparation method:

Cell culture supernatants from fibroblasts were collected with or without stimulus for Col1A2 production and centrifuged to remove any particulates and stored at <80C until used for the assay. 

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