COL1A2 ELISA Kit (Cattle) (OKCD04401)

Catalog No: OKCD04401

Size:96WELLS

Product Info

• Predicted Species Reactivity: Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-88%	85-93%	91-101%	78-93%
  EDTA Plasma (n=5)	78-89%	83-91%	85-94%	94-104%
  Heparin Plasma (n=5)	82-101%	89-96%	78-91%	79-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I Alpha 2 (COL1a2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type I Alpha 2 (COL1a2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type I Alpha 2 (COL1a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Collagen Type I Alpha 2 (COL1a2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 6.25-400ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average (%)
  Serum (n=5)	96-105	101
  EDTA Plasma (n=5)	94-104	101
  Heparin Plasma (n=5)	88-101	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type I Alpha 2 (COL1a2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type I Alpha 2 (COL1a2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Col1a2 Microplate: 96 Wells (12 x 8 Well strips)
  • Col1a2 Lyophilized Standard: 2
  • 100X Col1a2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 2.52 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: COL1A2
• NCBI Gene Id: 282188
• Protein Name: collagen alpha-2(I) chain
• Uniprot ID: P02465
• Protein Accession #: NP_776945.1
• Nucleotide Accession #: NM_174520.2