Catalog No: OKEH04797
Size:96 Wells
Price: $625.00
SKU
OKEH04797
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPig
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology col1a1 ELISA Kit (Pig) (OKEH04797) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for col1a1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for col1a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample col1a1 captured in well.
ELISA Kit Range1.56 - 100 ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
col1a1 Microplate96 Wells (12 x 8 Well Strips)
col1a1 Standard2
Sample Diluent1 x 20 mL
col1a1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity< 0.39 ng/mL
SpecificityThis assay recognizes recombinant and natural pig Collagen alpha-1(I) chain. No significant cross-reactivity or interference was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCOL1A1
NCBI Gene Id100733209
Protein NameType I collagen alpha 1 chain
Uniprot IDQ9MYT7
Protein Accession #XP_013004992.1 ?
Nucleotide Accession #XM_013149538.1
Protein Size (# AA)15
Molecular Weight2 kDa
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