COL1A1 ELISA Kit (Human) : 96 Wells (OKEH00542)

Catalog No: OKEH00542 (Formerly GWB-G8JPOW)

Size:96 Wells

Human COL1A1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology COL1A1 ELISA Kit (Human) (OKEH00542) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human COL1A1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human COL1A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human COL1A1 captured in well.
• ELISA Kit Range: 0.47 - 30ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.8% (n = 20)
  Mean Inter-assay CV%: < 7.2% (n = 20)
• ELISA Kit Component:
  • COL1A1 Microplate: 96 Wells (12 x 8 Well strips)
  • COL1A1 Lyophilized Standard: 2
  • 100X Biotinylated COL1A1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.117 ng/mL
• Specificity: Natural and recombinant Human Collagen alpha-1(I) chain
• Publications:

  Validating candidate biomarkers for different stages of non-alcoholic fatty liver disease. Medicine (Baltimore). 99, e21463 (2020). 32898995

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: COL1A1
• Gene Full Name: collagen type I alpha 1
• Alias Symbols: OI1, OI2, OI3, OI4, EDSC, CAFYD, EDSARTH1
• NCBI Gene Id: 1277
• Protein Name: Collagen alpha-1(I) chain
• Description of Target: Type I collagen is a member of group I collagen (fibrillar forming collagen).
• Uniprot ID: P02452
• Protein Accession #: NP_000079.2
• Nucleotide Accession #: NM_000088.3
• Protein Size (# AA): 1464
• Molecular Weight: 139 kDa