Catalog No: OKCD08016
Size:96WELLS
Price: $800.00
SKU
OKCD08016
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for COL17A1 ELISA Kit (Human) (OKCD08016) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type XVII (COL17). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type XVII (COL17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type XVII (COL17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Collagen Type XVII (COL17) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 3.12-200ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type XVII (COL17) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type XVII (COL17) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 1.13ng/mL |
Gene Symbol | COL17A1 |
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Gene Full Name | collagen type XVII alpha 1 chain |
Alias Symbols | 180 kDa bullous pemphigoid antigen 2;alpha 1 type XVII collagen;BA16H23.2;bA16H23.2 (collagen, type XVII, alpha 1 (BP180));BP180;BPA-2;BPAG2;Bullous pemphigoid antigen 2;bullous pemphigoid antigen 2 (180kD);collagen alpha-1(XVII) chain;collagen XVII, alpha-1 polypeptide;collagen, type XVII, alpha 1;ERED;LAD-1;type XVII collagen alpha-1. |
NCBI Gene Id | 1308 |
Protein Name | Collagen alpha-1(XVII) chain |
Description of Target | May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane. |
Uniprot ID | Q9UMD9 |
Protein Accession # | NP_000485.3 |
Nucleotide Accession # | NM_000494.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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