COL12A1 Chemi-Luminescent ELISA Kit (Human) (OKCD03388)

Catalog No: OKCD03388

Size:96WELLS

Human COL12A1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	88-102%	84-98%	96-103%	80-90%
  EDTA Plasma (n=5)	80-95%	91-104%	80-91%	89-97%
  Heparin Plasma (n=5)	79-90%	87-94%	80-102%	97-104%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Collagen Type XII (COL12). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Collagen Type XII (COL12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Collagen Type XII (COL12) level in the sample or standard.
• ELISA Kit Range: 27.4-20,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	87-94	91
  EDTA Plasma (n=5)	93-101	97
  Heparin Plasma (n=5)	86-103	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type XII (COL12) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type XII (COL12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • COL12A1 Microplate: 96 Wells (12 x 8 Well strips)
  • COL12A1 Lyophilized Standard: 2
  • 100X COL12A1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.312 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: COL12A1
• Gene Full Name: collagen type XII alpha 1 chain
• Alias Symbols: BA209D8.1;BTHLM2;COL12A1L;collagen alpha-1(XII) chain;collagen type XII proteoglycan;collagen, type XII, alpha 1;DJ234P15.1;EDSMYP;UCMD2.
• NCBI Gene Id: 1303
• Protein Name: Collagen alpha-1(XII) chain
• Description of Target: Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.
• Uniprot ID: Q99715
• Protein Accession #: NP_004361
• Nucleotide Accession #: NM_004370