Catalog No: OPCA03350
Price: $0.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for Cobra venom factor Recombinant Protein (Naja kaouthia) (OPCA03350)
Product Info
Predicted Species ReactivityMonocled cobra
Product FormatLiquid or Lyophilized powder
Reconstitution and StorageWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
PurificationAffinity purified using IMAC.
PurityGreater than 90% as determined by SDS-PAGE.
Storage BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
SourceE. Coli
Protein Range733-984 aa
TagN-terminal 6xHis-SUMO-tagged
ReferenceMolecular cloning and derived primary structure of cobra venom factor.Fritzinger D.C., Bredehorst R., Vogel C.-W.Proc. Natl. Acad. Sci. U.S.A. 91:12775-12779(1994)
WarningFor research use only.
Alias SymbolsComplement C3 homolog
Description of TargetComplement-activating protein in cobra venom. It is a structural and functional analog of complement component C3b, the activated form of C3. It binds factor B (CFB), which is subsequently cleaved by factor D (CFD) to form the bimolecular complex CVF/Bb. CVF/Bb is a C3/C5 convertase that cleaves both complement components C3 and C5. Structurally, it resembles the C3b degradation product C3c, which is not able to form a C3/C5 convertase. Unlike C3b/Bb, CVF/Bb is a stable complex and completely resistant to the actions of complement regulatory factors H (CFH) and I (CFI). Therefore, CVF continuously activates complement resulting in the depletion of complement activity.
Uniprot IDQ91132
Protein Size (# AA)733-984 aa
Molecular Weight44.4 kDa
Write Your Own Review
You're reviewing:Cobra venom factor Recombinant Protein (Monocled cobra) (OPCA03350)
Your Rating