CNTN1 ELISA Kit (Bovine) (OKEH08100)

Catalog No: OKEH08100

Size:96T

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology CNTN1 ELISA Kit (Bovine) (OKEH08100) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CNTN1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CNTN1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CNTN1 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 119% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.0% (n = 20)
  Mean Inter-assay CV%: <=5.7% (n = 20)
• ELISA Kit Component:
  • CNTN1 Microplate: 96 Wells (12 x 8 Well strips)
  • CNTN1 Lyophilized Standard: 2
  • 100X Biotinylated CNTN1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.
  Subcellular Location: Cell membrane Lipid-anchor GPI-anchor
  Unigene: Bt.349
  String: 9913.ENSBTAP00000042896
  KEGG: bta:281705
  Pfam: PF00041
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Bovine Contactin-1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CNTN1
• Gene Full Name: contactin 1
• Alias Symbols: contactin-1;neural cell surface protein F3.
• NCBI Gene Id: 281705
• Protein Name: Q28106
• Description of Target: Contactin-1
• Uniprot ID: https://www.uniprot.org/uniprot/Q28106
• Protein Accession #: NP_776705.1
• Nucleotide Accession #: NM_174280.2