Catalog No: OKCD03385
Size:96WELLS
Price: $800.00
SKU
OKCD03385
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for CNTN1 Chemi-Luminescent ELISA Kit (Human) (OKCD03385) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Chemiluminescent immunoassay | ||||||||||||||||||||||
ELISA Kit Detection Method | Chemiluminescent | ||||||||||||||||||||||
ELISA Kit Duration | 2h, 40min | ||||||||||||||||||||||
ELISA Kit Principle | The microplate provided in this kit has been pre-coated with an antibody specific to Contactin 1 (CNTN1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Contactin 1 (CNTN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Contactin 1 (CNTN1) level in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 13.7-10,000pg/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Contactin 1 (CNTN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Contactin 1 (CNTN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates and other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.156 ng/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | CNTN1 |
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Gene Full Name | contactin 1 |
Alias Symbols | contactin-1;F3;glycoprotein gP135;GP135;MYPCN;neural cell surface protein F3. |
NCBI Gene Id | 1272 |
Protein Name | Contactin-1 |
Description of Target | Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth (By similarity). |
Uniprot ID | Q12860 |
Protein Accession # | NP_001242992 |
Nucleotide Accession # | NM_001256063 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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