Catalog No: OKCD03385
Size:96WELLS
Price: $800.00
SKU
OKCD03385
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CNTN1 Chemi-Luminescent ELISA Kit (Human) (OKCD03385)

Datasheets/ManualsPrintable datasheet for CNTN1 Chemi-Luminescent ELISA Kit (Human) (OKCD03385)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Contactin 1 (CNTN1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Contactin 1 (CNTN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Contactin 1 (CNTN1) level in the sample or standard.
ELISA Kit Range13.7-10,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Contactin 1 (CNTN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Contactin 1 (CNTN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CNTN1 Microplate96 Wells (12 x 8 Well strips)
CNTN1 Lyophilized Standard2
100X CNTN1 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity0.156 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCNTN1
Gene Full Namecontactin 1
Alias Symbolscontactin-1;F3;glycoprotein gP135;GP135;MYPCN;neural cell surface protein F3.
NCBI Gene Id1272
Protein NameContactin-1
Description of TargetContactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth (By similarity).
Uniprot IDQ12860
Protein Accession #NP_001242992
Nucleotide Accession #NM_001256063
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