CNTF ELISA Kit (Rat) (OKBB00365)

Catalog No: OKBB00365

Size:96 Wells

Rat CNTF ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's rat CNTF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CNTF has been precoated onto 96-well plates. Standards(E.coli, A2-M200) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CNTF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat CNTF amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	107	452	1287	193	643	1057
  SD	4.8	18.9	50.2	16.2	52.7	77.2
  CV%	4.5	4.2	3.9	8.4	8.2	7.3

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Rat CNTF Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Rat CNTF Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Rat CNTF Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant rat CNTF
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: A2-M200
• Description: For quantitative detection of rat CNTF in cell culture supernatants, serum and plasma(heparin, EDTA, citrate).

Target Info

• Gene Symbol: CNTF
• Gene Full Name: ciliary neurotrophic factor
• Alias Symbols: AI429687, Ciliary Neurotrophic Factor, HCNTF, MGC41235, OTTHUMP00000174731, OTTMUSP00000029848
• NCBI Gene Id: 25707
• Protein Name: Ciliary neurotrophic factor
• Description of Target: Ciliary neurotrophic factor (CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The hCNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid (aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85% homology with the deduced aa sequence encoding hCNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.
• Uniprot ID: P20294
• Protein Accession #: NP_037298.1
• Nucleotide Accession #: NM_013166.1