Catalog No: OKCD02073 (Formerly GWB-KBBRP7)
Size:96WELLS
Price: $750.00
SKU
OKCD02073
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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- Email: info@avivasysbio.com
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Datasheets/Manuals | Printable datasheet for Cnpy2 ELISA Kit (Mouse) (OKCD02073) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Canopy 2 Homolog (CNPY2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Canopy 2 Homolog (CNPY2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Canopy 2 Homolog (CNPY2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Canopy 2 Homolog (CNPY2) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Canopy 2 Homolog (CNPY2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Canopy 2 Homolog (CNPY2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.055 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Canopy 2 Homolog (CNPY2). No significant cross-reactivity or interference between Canopy 2 Homolog (CNPY2) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | Cnpy2 |
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Gene Full Name | canopy FGF signaling regulator 2 |
Alias Symbols | 5330432A10Rik;AW229003;canopy 2 homolog;D10Bwg1546e;MIR-interacting saposin-like protein;protein canopy homolog 2;putative secreted protein ZSIG9;Tmem4;transmembrane protein 4;Zsi;Zsig9. |
NCBI Gene Id | 56530 |
Protein Name | Protein canopy homolog 2 |
Description of Target | Positive regulator of neurite outgrowth by stabilizing myosin regulatory light chain (MRLC). It prevents MIR-mediated MRLC ubiquitination and its subsequent proteasomal degradation (By similarity). |
Uniprot ID | Q9QXT0 |
Protein Accession # | NP_064337.1 |
Nucleotide Accession # | NM_019953.1 |
Protein Size (# AA) | 182 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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