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Catalog No: OKEH08627
Size:96T
Price: $725.00
SKU
OKEH08627
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology CNNM4 ELISA Kit (Rat) (OKEH08627) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CNNM4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CNNM4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CNNM4 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 84% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=5.4% (n = 20)
Mean Inter-assay CV%: <=6.4% (n = 20)
ELISA Kit Component
ComponentAmount
CNNM4 Microplate96 Wells (12 x 8 Well strips)
CNNM4 Lyophilized Standard2
100X Biotinylated CNNM4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Probable metal transporter. The interaction with the metal ion chaperone COX11 suggests that it may play a role in sensory neuron functions. May play a role in biomineralization and retinal function.
::Subcellular Location: Cell membrane Multi-pass membrane protein
::Unigene: Rn.857
SMR: P0C588
String: 10116.ENSRNOP00000021434
::KEGG: rno:363216
Pfam: PF00571
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.086ng/mL
SpecificityNatural and recombinant Rat Metal transporter CNNM4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCnnm4
Gene Full Namecyclin and CBS domain divalent metal cation transport mediator 4
Alias Symbolsancient conserved domain-containing protein 4;cyclin-M4;metal transporter CNNM4.
NCBI Gene Id363216
Protein NameP0C588
Description of TargetMetal transporter CNNM4
Uniprot IDhttps://www.uniprot.org/uniprot/P0C588
Protein Accession #NP_001257979.1
Nucleotide Accession #NM_001271050.1
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