CNNM2 ELISA Kit (Rat) (OKEH08628)

Catalog No: OKEH08628

Size:96T

Rat CNNM2 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology CNNM2 ELISA Kit (Rat) (OKEH08628) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CNNM2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CNNM2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CNNM2 captured in well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.0% (n = 20)
  Mean Inter-assay CV%: <=9.6% (n = 20)
• ELISA Kit Component:
  • CNNM2 Microplate: 96 Wells (12 x 8 Well strips)
  • CNNM2 Lyophilized Standard: 2
  • 100X Biotinylated CNNM2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Divalent metal cation transporter. Mediates transport of divalent metal cations in an order of Mg(2+) > Co(2+) > Mn(2+) > Sr(2+) > Ba(2+) > Cu(2+) > Fe(2+).
  Subcellular Location: Cell membrane Multi-pass membrane protein
  Unigene: Rn.205139
  SMR: Q5U2P1
  String: 10116.ENSRNOP00000051583
  KEGG: rno:294014
  Pfam: PF00571
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 14.4pg/mL
• Specificity: Natural and recombinant Rat Metal transporter CNNM2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cnnm2
• Gene Full Name: cyclin and CBS domain divalent metal cation transport mediator 2
• Alias Symbols: ancient conserved domain-containing protein 2;cyclin-M2;metal transporter CNNM2.
• NCBI Gene Id: 294014
• Protein Name: Q5U2P1
• Description of Target: Metal transporter CNNM2
• Uniprot ID: https://www.uniprot.org/uniprot/Q5U2P1
• Protein Accession #: NP_001011942.1
• Nucleotide Accession #: NM_001011942.1