CNDP1 ELISA Kit (Human) (OKEH00851)

Catalog No: OKEH00851

Size:96 Wells

Human CNDP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CNDP1 ELISA Kit (Human) (OKEH00851) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CNDP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CNDP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CNDP1 captured in well.
• ELISA Kit Range: 1.56 - 100ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.2% (n = 20)
  Mean Inter-assay CV%: < 6.6% (n = 20)
• ELISA Kit Component:
  • CNDP1 Microplate: 96 Wells (12 x 8 Well strips)
  • CNDP1 Lyophilized Standard: 2
  • 100X Biotinylated CNDP1 Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.056 ng/mL
• Specificity: Natural and recombinant Human Beta-Ala-His dipeptidase
• Publications:

  Quantitative Comparative Proteomics Reveals Candidate Biomarkers for the Early Prediction of Gestational Diabetes Mellitus: A Preliminary Study. In Vivo. 34, 517-525 (2020). 32111749

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CNDP1
• Gene Full Name: carnosine dipeptidase 1 (metallopeptidase M20 family)
• Alias Symbols: CN1, CPGL2, HsT2308
• NCBI Gene Id: 84735
• Protein Name: Beta-Ala-His dipeptidase
• Description of Target: This gene encodes a member of the M20 metalloprotease family. The encoded protein is specifically expressed in the brain, is a homodimeric dipeptidase which was identified as human carnosinase. This gene contains trinucleotide (CTG) repeat length polymorphism in the coding region.
• Uniprot ID: Q96KN2
• Protein Accession #: NP_116038.4
• Nucleotide Accession #: NM_032649.5
• Protein Size (# AA): 507
• Molecular Weight: 57 kDa