Catalog No: OKEH00851
Size:96 Wells
Price: $525.00
SKU
OKEH00851
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CNDP1 ELISA Kit (Human) (OKEH00851) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CNDP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CNDP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CNDP1 captured in well.
ELISA Kit Range1.56 - 100ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 6.6% (n = 20)
ELISA Kit Component
ComponentAmount
CNDP1 Microplate96 Wells (12 x 8 Well strips)
CNDP1 Lyophilized Standard2
100X Biotinylated CNDP1 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.056 ng/mL
SpecificityNatural and recombinant Human Beta-Ala-His dipeptidase
Publications

Quantitative Comparative Proteomics Reveals Candidate Biomarkers for the Early Prediction of Gestational Diabetes Mellitus: A Preliminary Study. In Vivo. 34, 517-525 (2020). 32111749

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCNDP1
Gene Full Namecarnosine dipeptidase 1 (metallopeptidase M20 family)
Alias SymbolsCN1, CPGL2, HsT2308
NCBI Gene Id84735
Protein NameBeta-Ala-His dipeptidase
Description of TargetThis gene encodes a member of the M20 metalloprotease family. The encoded protein is specifically expressed in the brain, is a homodimeric dipeptidase which was identified as human carnosinase. This gene contains trinucleotide (CTG) repeat length polymorphism in the coding region.
Swissprot IdQ96KN2
Protein Accession #NP_116038.4
Nucleotide Accession #NM_032649.5
Protein Size (# AA)507
Molecular Weight57 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CNDP1.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CNDP1.
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