Catalog No: OKCD08784
Size:96 Wells
Price: $800.00
SKU
OKCD08784
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CNDP1 ELISA Kit (Human) (OKCD08784) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CNDP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CNDP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CNDP1 captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CNDP1. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CNDP1 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
CNDP1 Microplate96 Wells (12 x 8 Well strips)
CNDP1 Lyophilized Standard2
100X CNDP1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, cerebrospinal fluid and other biological fluids
Sensitivity< 0.064ng/mL
Gene SymbolCNDP1
Gene Full Namecarnosine dipeptidase 1
Alias SymbolsCN1, CPGL2, HsT2308
NCBI Gene Id84735
Protein NameBeta-Ala-His dipeptidase
Description of TargetCNDP1 is a member of the M20 metalloprotease family. The protein is specifically expressed in the brain, is a homodimeric dipeptidase which was identified as human carnosinase. CNDP1 gene contains trinucleotide (CTG) repeat length polymorphism in the coding region.
Swissprot IdQ96KN2
Protein Accession #NP_116038.4
Nucleotide Accession #NM_032649.5
Tissue ToolFind tissues and cell lines supported by RNA-seq analysis to express CNDP1.
RNA SeqFind tissues and cell lines supported by DNA array analysis to express CNDP1.
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