Catalog No: OONB00073
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CMV Antigen (OONB00073)
A concentrated source of human Cytomegalovirus particles purified by sucrose gradient ultracentrifugation.
Product Format | Liquid 0.2% Triton X-100, 10mM Tris-HCl, 1mM EDTA and 100mM NaCl (pH 7.8) |
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Host | Human Foreskin Fibroblasts (HFF) |
Additional Information | Strain: AD-169 Inactivation: Purified virus particles were lysed in 0.2% Triton X-100 in STE buffer. The lysate was tested by passage on permissive cells for 2 weeks yielding no CPE. |
Reconstitution and Storage | Store frozen at less than -20°C; preferably at -80°C. Avoid freeze/thaw cycles. Purified enveloped viruses can aggregate upon freezing/thawing, so homogenization by vortexing or sonication is recommended before use. |
Source | Native |
Concentration | Adjusted to 1 mg/ml (BCA assay) |
Purification | Purified by ultracentrifugation. |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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