CMTM6 Chemi-Luminescent ELISA Kit (Human) (OKCD03377)

Catalog No: OKCD03377

Size:96WELLS

Human CMTM6 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	92-105%	86-96%	97-105%	80-99%
  EDTA Plasma (n=5)	90-98%	81-101%	97-105%	82-99%
  Heparin Plasma (n=5)	84-103%	81-91%	87-94%	86-103%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Chemokine Like Factor Superfamily 6 (CKLFSF6). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chemokine Like Factor Superfamily 6 (CKLFSF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chemokine Like Factor Superfamily 6 (CKLFSF6) level in the sample or standard.
• ELISA Kit Range: 6.9-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-99	88
  EDTA Plasma (n=5)	95-102	98
  Heparin Plasma (n=5)	97-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine Like Factor Superfamily 6 (CKLFSF6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine Like Factor Superfamily 6 (CKLFSF6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CMTM6 Microplate: 96 Wells (12 x 8 Well strips)
  • CMTM6 Lyophilized Standard: 2
  • 100X CMTM6 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 6.9 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CMTM6
• Gene Full Name: CKLF like MARVEL transmembrane domain containing 6
• Alias Symbols: chemokine-like factor super family 6;chemokine-like factor superfamily 6;chemokine-like factor superfamily member 6;CKLF-like MARVEL transmembrane domain-containing protein 6;CKLFSF6;PRO2219.
• NCBI Gene Id: 54918
• Protein Name: CKLF-like MARVEL transmembrane domain-containing protein 6
• Description of Target: Master regulator of recycling and plasma membrane expression of PD-L1/CD274, an immune inhibitory ligand critical for immune tolerance to self and antitumor immunity. Associates with both constitutive and IFNG-induced PD-L1/CD274 at recycling endosomes, where it protects PD-L1/CD274 from being targeted for lysosomal degradation, likely by preventing its STUB1-mediated ubiquitination. May stabilize PD-L1/CD274 expression on antigen presenting cells and potentiates inhibitory signaling by PDCD1/CD279, its receptor on T-cells, ultimately triggering T-cell anergy.
• Uniprot ID: Q9NX76
• Protein Accession #: NP_060271
• Nucleotide Accession #: NM_017801