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Catalog No: OKEH03335
Size:96T
Price: $675.00
SKU
OKEH03335
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH03335
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CMKLR1 ELISA Kit (Human) (OKEH03335) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CMKLR1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for CMKLR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CMKLR1 captured in well.
ELISA Kit Range78-5000pg/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 7.6% (n = 20)
Mean Inter-assay CV%: <= 11.0% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CMKLR1 Microplate96 Wells (12 x 8 Well strips)
CMKLR1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CMKLR1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity39.4 pg/mL
SpecificityNatural and recombinant Human Chemokine-like receptor 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCMKLR1
Gene Full Namechemerin chemokine-like receptor 1
Alias Symbolschemerin receptor;CHEMERINR;chemokine receptor-like 1;chemokine-like receptor 1;ChemR23;DEZ;G-protein coupled receptor ChemR23;G-protein coupled receptor DEZ;orphan G-protein coupled receptor, Dez;resolvin E1 receptor;RVER1.
NCBI Gene Id1240
Protein NameQ99788
Description of TargetChemokine-like receptor 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q99788
Protein Accession #NP_001135815.1
Nucleotide Accession #NM_001142343.1
Protein Size (# AA)373
Molecular Weight42 kDa
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