Catalog No: OPPA01606 (Formerly GWB-BSP007)
Size:2UG
Price: $75.00
SKU
OPPA01606
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

Clusterin Protein (OPPA01606)

Datasheets/ManualsPrintable datasheet for OPPA01606
Product Info
Predicted Species ReactivityDog
Product FormatCanine Clusterin was lyophilized from PBS, pH 7.5. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
HostE. Coli
Additional InformationSolubility: Add deionized H2O to a working volume of 0.5mg/ml and let the lyophilized pellet dissolve completely.
::Product Introduction: Clusterin also named Apoliprotein J (APO-J) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified.The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the a and b chains. These are assembled in anti-parallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil a-helices and three predicted amphipathic a-helices.Across a broad range of species clusterin shows a high degree of sequence homology ranging from 70% to 80%. It is nearly ubiquitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen.It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodeling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition.A genuine function of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced insults caused by degraded and misfolded protein precipitates.Clusterin is up- or down regulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.
Product Description: Apoliprotein-J canine Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 433 amino acids and having a molecular mass of 50.6 kDa. The protein is fused to His tag at N-Terminus.The Apoliprotein-J canine is purified by proprietary chromatographic techniques. The amino acids sequence is identical to UniProtKB/Swiss-Prot entry P25473 amino acids 23 - 445.
Reconstitution and StorageStore lyophilized protein at -20C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4C for a limited period of time; it does not show any change after two weeks at 4C. The lyophilized protein remains stable until the expiry date when stored at -20C.
PurityGreater than 95% as determined by SDS PAGE.
Peptide SequenceMKHHHHHHAS DQAVSDTELQ EMSTEGSKYI NKEIKNALKG VKQIKTLIEQ TNEERKSLLS NLEEAKKKKE DALNDTKDSE TKLKASQGVC NDTMMALWEE CKPCLKQTCM KFYARVCRSG SGLVGHQLEE FLNQSSPFYF WMNGDRIDSL LENDRQQTHA LDVMQDSFNR ASSIMDELFQ DRFFTREPQD TYHYSPFSLF QRRPFFNPKF RIARNIIPFP RFQPLNFHDM FQPFFDMIHQ AQQAMDVNLH RIPYHFPIEF PEEDNRTVCK EIRHNSTGCL KMKDQCEKCQ EILSVDCSSN NPAQVQLRQE LSNSLQIAEK FTKLYDELLQ SYQEKMFNTS SLLKQLNEQF SWVSQLANLT QSEDPFYLQV TTVGSQTSDS NVPVGFTKVV VKLFDSDPIT VMIPEAVSRN NPKFMETVAE KALQEYRQKHREE.
Gene Symbolk9Clusterin
Alias SymbolsGP80
NCBI Gene Id442971
Protein NameClusterin
Description of TargetRecombinant Canine Apoliprotein-J
Uniprot IDP25473
Protein Accession #NP_001003370.1
Protein Size (# AA)Recombinant
Write Your Own Review
You're reviewing:Clusterin Protein (OPPA01606)
Your Rating