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Catalog No: OKIA00138 (Formerly GWB-R374CL)
Size:1x96WELLPLATE
Price: $525.00
SKU
OKIA00138
Availability: Domestic: within 1 week delivery | International: 1 week
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
Product FormatIn Solution
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration100 Minutes
ELISA Kit PrincipleAviva Systems Biology Clusterin ELISA Kit (Rat) (OKIA00138) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Clusterin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Clusterin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Clusterin captured in the well.
ELISA Kit Range12.5 ng/ml - 800 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Rat Clusterin (Apolipoprotein J) Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Rat Clusterin (Apolipoprotein J) Calibrator1
Additional InformationIntended Use: The CLUSTERIN test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring CLUSTERIN in biological samples of Rat.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Rat CLUSTERIN antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-RAT CLUSTERIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat CLUSTERIN.
7. RAT CLUSTERIN CALIBRATOR
One vial containing a Rat CLUSTERIN calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity3.399 ng/ml
Predicted Homology Based on Immunogen SequenceRat
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCLU
Gene Full Nameclusterin
Alias SymbolsCLI, DAG, APOJ, SGP2, SP40, SGP-2, SP-40, Trpm2, Trpmb, TRPM-2, TRPM2B, RATTRPM2B
NCBI Gene Id24854
Protein NameClusterin
Description of TargetFunctions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Promotes apoptosis when in the nucleus. Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation.
Uniprot IDP05371
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