Clusterin Antibody (OAPA00164)

Catalog No: OAPA00164 (Formerly GWB-BSP770)

Size:5UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Clonality: Monoclonal
• Clone: P1A11AT.
• Isotype: Mouse IgG1 heavy chain and kappa light chain.
• Host: Mouse
• Application: WB, IHC
  Product Type: Mouse Anti Human Monoclonal.
  Product Introduction: Clusterin also named Apoliprotein J (APO-J) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified. The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the alpha and beta chains. These are assembled in anti-parallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil alpha -helices and three predicted amphipathic a-helices. Across a broad range of species clusterin shows a high degree of sequence homology ranging from 70% to 80%. It is nearly ubiquitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, b
• Reconstitution and Storage: For periods up to 1 month store at 4C, for longer periods of time, store at -20C. Prevent freeze thaw cycles.
  Stability: 12 months at -20C. 1 month at 4C.
• Immunogen: The immunogen for anti-Clusterin antibody: anti-human Clusterin mAb, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with Recombinant human Clusterin amino acids 1-333 purified from E. coli.
• Purity: Clusterin antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Application Info: Clusterin antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500 ~ 1:2,000 and immunohistochemistry analysis is 1:100~200. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:100.

Target Info

• Gene Symbol: Clusterin
• Alias Symbols: CLI, DAG, APOJ, SGP2, SP40, SGP-2, SP-40, Trpm2, Trpmb, TRPM-2, TRPM2B, RATTRPM2B
• NCBI Gene Id: 24854
• Protein Name: Clusterin
• Description of Target: Mouse Anti Human Clusterin
• Uniprot ID: P05371
• Protein Accession #: NP_444180.2