Catalog No: OKCD07191
Size:96 Wells
Price: $825.00
SKU
OKCD07191
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CLU ELISA Kit (Rat) (OKCD07191) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLU has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLU is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLU captured in the well.
ELISA Kit Range6.25-400ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CLU. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CLU using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
CLU Microplate96 Wells (12 x 8 Well strips)
CLU Lyophilized Standard2
100X CLU HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 2.37ng/mL
Gene SymbolCLU
Gene Full Nameclusterin
Alias SymbolsCLI, DAG, APOJ, SGP2, SP40, SGP-2, SP-40, Trpm2, Trpmb, TRPM-2, TRPM2B, RATTRPM2B
NCBI Gene Id24854
Protein Nameclusterin
Description of TargetThe protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders.
Swissprot IdP05371
Protein Accession #NP_444180.2
Nucleotide Accession #NM_053021.2
Tissue ToolFind tissues and cell lines supported by RNA-seq analysis to express CLU.
RNA SeqFind tissues and cell lines supported by DNA array analysis to express CLU.
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