CLU Antibody - C-terminal region (ARP61142_P050)

Catalog No: ARP61142_P050

CLU antibody - C-terminal region (ARP61142_P050) validated by WB using Fetal Brain Lysate at 1ug/ml.

Lanes:
1. 40 ug human HK2 cell (kidney proximal tubular cell line) lysate
2. 40 ug H2O2 treated human HK2 Cell lysate
3. 40 ug H2O2 treated human HK2 Cell lysate
4. 40 ug H2O2 treated human HK2 Cell lysate
5. 40 ug H2O2 treated human HK2 Cell lysate
6. 40 ug H2O2 treated human HK2 Cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Goat anti-Rabbit HRP
Secondary Antibody Dilution:
1:2000
Gene Name:
CLU
Submitted by:
The University of Queensland, School of Medicine, Centre for Kidney Disease Research

Rabbit Anti-CLU Antibody
Catalog Number: ARP61142_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Cytoplasm in hepatocytes
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Rabbit Anti-CLU Antibody
Catalog Number: ARP61142_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Product Info

• Tested Species Reactivity: Human, Horse
• Predicted Species Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CLU
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Guinea Pig: 86%; Horse: 100%; Human: 100%; Mouse: 86%; Rabbit: 91%; Rat: 86%
• Peptide Sequence: Synthetic peptide located within the following region: CREILSVDCSTNNPSQAKLRRELDESLQVAERLTRKYNELLKSYQWKMLN
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-CLU (ARP61142_P050) antibody is Catalog # AAP61142 (Previous Catalog # AAPP47252)
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Publications:

  Clusterin secretion is attenuated by the proinflammatory cytokines interleukin-1β and tumor necrosis factor-α in models of cartilage degradation. J Orthop Res. (2020) 32725904

Target Info

• Gene Symbol: CLU
• Gene Full Name: Clusterin
• Alias Symbols: CLI, AAG4, APOJ, CLU1, CLU2, KUB1, SGP2, APO-J, SGP-2, SP-40, TRPM2, TRPM-2, NA1/NA2
• NCBI Gene Id: 1191
• Protein Name: Clusterin
• Description of Target: The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.
• Uniprot ID: P10909
• Protein Accession #: NP_001822
• Nucleotide Accession #: NM_001831
• Protein Size (# AA): 501
• Molecular Weight: 58kDa
• Protein Interactions: HYOU1; HSP90B1; HSPA5; BAX; TUFM; NUCB2; HSPA1L; TUBB6; MDM2; FOS; CDK19; APOA1; KEAP1; POLR1C; RPL23; KLF11; BAG6; RBBP8; MAPK9; PDIA3; CYP2E1; BCL2L1; TSC22D4; MDC1; LIG4; PAXIP1; CLUAP1; IL24; PRNP; PON1; HSPD1; APP; FBXO6; LYZ; LRP2; HBA1; XRCC6; ELN;