Catalog No: OKEH01658
Size:96T
Price: $525.00
SKU
OKEH01658
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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CLPP ELISA Kit (Human) : 96 Wells (OKEH01658)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CLPP ELISA Kit (Human) (OKEH01658) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human CLPP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CLPP and incubated. The CLPP found in the sample or standards competes with the biotinylated CLPP for limited binding sites on the immobilized anti-human CLPP antibody. Excess unbound biotinylated CLPP and sample or standard CLPP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CLPP captured in the well and inversely proportional to the amount of CLPP which was contained in the sample or standard.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.2% (n = 20)
Mean Inter-assay CV%: < 10.6% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CLPP Microplate96 Wells (12 x 8 Well strips)
CLPP Lyophilized Standard2
Sample Diluent1 x 20 mL
100X CLPP-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.054 ng/mL
SpecificityNatural and recombinant Human ATP-dependent Clp protease proteolytic subunit, mitochondrial
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolCLPP
Gene Full Namecaseinolytic mitochondrial matrix peptidase proteolytic subunit
Alias SymbolsATP-dependent Clp protease proteolytic subunit, mitochondrial;ATP-dependent protease ClpAP, proteolytic subunit, human;ClpP caseinolytic peptidase ATP-dependent, proteolytic subunit;ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog;ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog;DFNB81;endopeptidase Clp;PRLTS3;putative ATP-dependent Clp protease proteolytic subunit, mitochondrial.
NCBI Gene Id8192
Protein NameQ16740
Description of TargetATP-dependent Clp protease proteolytic subunit, mitochondrial
Uniprot IDhttps://www.uniprot.org/uniprot/Q16740
Protein Accession #NP_006003.1
Nucleotide Accession #NM_006012.2
Protein Size (# AA)277
Molecular Weight30 kDa
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