CLPP Antibody (OAGA00331)

Catalog No: OAGA00331

Size:100 ul

Sample(30 ?g of whole cell lysate)
A:293T
B:A431
C:H1299
10% SDS PAGE
OAGA00331 diluted at 1:1500

Immunohistochemical analysis of paraffin-embedded H520 xenograft, using CLPP(OAGA00331) antibody at 1:100 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HepG2, Molt-4, Raji
  Cellular Localization: Mitochondrion
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide corresponding to a region within amino acids 215 and 277 of CLPP (Uniprot ID#Q16740)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: CLPP
• Gene Full Name: Putative ATP-dependent Clp protease proteolytic subunit, mitochondrial
• Alias Symbols: DFNB81, PRLTS3
• NCBI Gene Id: 8192
• Protein Name: Putative ATP-dependent Clp protease proteolytic subunit, mitochondrial
• Uniprot ID: Q16740
• Protein Accession #: NP_006003.1
• Molecular Weight: 30 kDa