CLPB ELISA Kit (Human) (OKWB00210)

Catalog No: OKWB00210

Size:96T

Human CLPB ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	86-99%	91-97%	85-99%	85-104%
  EDTA Plasma (n=5)	82-100%	82-100%	95-100%	83-95%
  Heparin Plasma (n=5)	81-99%	80-98%	81-98%	89-100%

• ELISA Kit Principle: Aviva Systems Biology CLPB ELISA Kit (Human) (OKWB00210) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLPB has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLPB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLPB captured in the well.
• ELISA Kit Range: 0.781-50ng/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	86-98	94
  EDTA Plasma (n=5)	86-104	95
  Heparin Plasma (n=5)	93-103	98

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • CLPB Microplate: 96 Wells (12 x 8 Well strips)
  • CLPB Lyophilized Standard: 2 x 50 ng
  • 100X CLPB Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 0.469 ng/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CLPB
• Gene Full Name: caseinolytic mitochondrial matrix peptidase chaperone subunit B
• Alias Symbols: ANKCLB;ankyrin-repeat containing bacterial clp fusion;caseinolytic peptidase B protein homolog;ClpB caseinolytic peptidase B homolog;ClpB homolog, mitochondrial AAA ATPase chaperonin;HSP78;MEGCANN;MGCA7;SKD3;suppressor of potassium transport defect 3;testicular secretory protein Li 11.
• NCBI Gene Id: 81570
• Protein Name: Caseinolytic peptidase B protein homolog
• Description of Target: May function as a regulatory ATPase and be related to secretion/protein trafficking process.
• Uniprot ID: Q9H078
• Protein Accession #: NP_001245321
• Nucleotide Accession #: NM_001258392