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CLK1 Antibody - N-terminal region (ARP52021_P050)

100 ul
$319.00
In Stock
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Conjugation Options

ARP52021_P050-FITC Conjugated

ARP52021_P050-HRP Conjugated

ARP52021_P050-Biotin Conjugated

Gene Symbol:
CLK1
Official Gene Full Name:
CDC-like kinase 1
NCBI Gene Id:
1195
Protein Name:
Dual specificity protein kinase CLK1
Swissprot Id:
P49759
Protein Accession #:
NP_004062
Nucleotide Accession #:
NM_004071
Alias Symbols:
CLK, CLK/STY, STY
Replacement Item:
This antibody may replace item sc-113676 from Santa Cruz Biotechnology.
Description of Target:
This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. In the nucleus, the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine rich proteins. Therefore, the encoded protein may play an indirect role in governing splice site selection. Multiple transcript variants encoding different isoforms have been found for this gene.
Protein Size (# AA):
484
Molecular Weight:
57kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Affinity Purified
Application:
WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CLK1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CLK1.
Immunogen:
The immunogen is a synthetic peptide directed towards the N terminal region of human CLK1
Predicted Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Tested Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 86%; Rabbit: 100%; Rat: 85%
Complete computational species homology data:
Anti-CLK1 (ARP52021_P050)
Peptide Sequence:
Synthetic peptide located within the following region: HSSAQENKRCKYNHSKMCDSHYLESRSINEKDYHSRRYIDEYRNDYTQGC
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
KRTAP10-7; PDE9A; PRMT5; SRPK2; SRPK1; CLK1; BAG3; PIAS4; ISG15; UBC; YWHAG; LUC7L3; PPIG; SRSF6; PRPF4; GOLM1; UBL5; SRSF1; PTPN1; MBP; BCLAF1; NCOR1; SRSF3; SAFB;
Blocking Peptide:
For anti-CLK1 (ARP52021_P050) antibody is Catalog # AAP52021 (Previous Catalog # AAPP44291)
Datasheets/Manuals:
Printable datasheet for anti-CLK1 (ARP52021_P050) antibody
Sample Type Confirmation:

CLK1 is supported by BioGPS gene expression data to be expressed in HEK293T

Publications:

Aubol, BE; Wu, G; Keshwani, MM; Movassat, M; Fattet, L; Hertel, KJ; Fu, XD; Adams, JA; Release of SR Proteins from CLK1 by SRPK1: A Symbiotic Kinase System for Phosphorylation Control of Pre-mRNA Splicing. 63, 218-28 (2016). WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat 27397683

Eisenreich, A. et al. Regulation of pro-angiogenic tissue factor expression in hypoxia-induced human lung cancer cells. Oncol. Rep. 30, 462-70 (2013). WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat 23604472

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1 Item(s)

122/05/2019 23:30
  • Overall Experience:
  • Quality:
Human breast cancer (MCF7) cell line in WB

Submitted by:
Jing Chen
Dept. of Pharmacology & Cancer Biology, Duke University

CLK1

“The CLK N terminus antibody gives stronger signal of the band around 56 KD and a cleaner background. I think there should be no problem for WB if lowering the dilution to 1:1000”

Sample type: Human breast cancer (MCF7) cell line

Lane description: 20ug MCF7 lysate

Primary antibody dilution: 1:500 dilution

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