CLIC4 ELISA Kit (Human) (OKCD01213)

Catalog No: OKCD01213 (Formerly GWB-KBBSK8)

Size:96WELLS

Human CLIC4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chloride Intracellular Channel Protein 4 (CLIC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chloride Intracellular Channel Protein 4 (CLIC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chloride Intracellular Channel Protein 4 (CLIC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chloride Intracellular Channel Protein 4 (CLIC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chloride Intracellular Channel Protein 4 (CLIC4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chloride Intracellular Channel Protein 4 (CLIC4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CLIC4 Microplate: 96 Wells (12 x 8 Well Strips)
  • CLIC4 Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated CLIC4 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 30 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chloride Intracellular Channel Protein 4 (CLIC4). No significant cross-reactivity or interference between Chloride Intracellular Channel Protein 4 (CLIC4) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CLIC4
• Gene Full Name: chloride intracellular channel 4
• Alias Symbols: chloride intracellular channel 4 like;chloride intracellular channel protein 4;CLIC4L;epididymis secretory sperm binding protein;H1;huH1;intracellular chloride ion channel protein p64H1;MTCLIC;p64H1.
• NCBI Gene Id: 25932
• Protein Name: Chloride intracellular channel protein 4
• Description of Target: Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).
• Uniprot ID: Q9Y696
• Protein Accession #: NP_039234
• Nucleotide Accession #: NM_013943.2
• Protein Size (# AA): 253