Clenbuterol ELISA Kit (OKAO00117)

Catalog No: OKAO00117

Size:96 Wells

Product Info

• Predicted Species Reactivity: General
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology Clenbuterol ELISA Kit (OKAO00117) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Clenbuterol has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Clenbuterol antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
• ELISA Kit Range: 0.05-4.05 ppb
• ELISA Kit Recovery:

  Sample	Amount
  Urine	95% +/- 10%
  Tissue	85% +/- 15%
  Feed	85% +/- 15%

• ELISA Kit Reproducibility: Mean Intra-Assay: CV<8% (n= 3 x 20)
  Mean Inter-Assay: CV<12% (n= 3 x 40)
• ELISA Kit Component:
  • Clenbuterol Microplate: 96 Wells (12 x 8 Well Strips)
  • Clenbuterol Standards: 6 x 1 mL
  • Clenbuterol Rabbit Detector Antibody: 1 x 6 mL
  • Anti-Rabbit / HRP Conjugate: 1 x 6 mL
  • Detection Reagent A: 1 x 6 mL
  • Detection Reagent B: 1 x 6 mL
  • Stop Solution: 1 x 6 mL
  • 20X Wash Buffer: 1 x 20 mL
  • 10X Sample Prep Buffer: 1 x 20 mL
• Additional Information: CAS: 93106-60-6
  PubChem: 2783
  Molecular Formula: C12H18Cl2N2O
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Urine, tissue, feed
• Sensitivity:

  Sample	Amount
  Urine	0.05 ppb
  Tissue (1)	0.2 ppb
  Tissue (2)	0.05 ppb
  Feed	0.5 ppb

• Specificity:

  Substance	Cross Reactivity Rate
  Clenbuterol	100%
  Terbutalin	1%
  Mabuterol	1%
  Brombuterol	1%
  Salbutamal	1%
  Ractopamine	1%

• Assay Info: Assay Methodology: Competitive Inhibition ELISA

Target Info

• Alias Symbols: CLE, Clenbuterol, Clenbuterolum, 37148-27-9, Monores, NAB-365, Clenbuterolum
• Protein Name: Clenbuterol
• Description of Target: Clenbuterol is a substituted phenylaminoethanol that has beta-2 adrenomimetic properties at very low doses. It is used as a bronchodilator in asthma.
• Molecular Weight: 277.19012 g/mol