CLEC3B ELISA Kit (Human) (OKCD09303)

Catalog No: OKCD09303

Size:96WELLS

CLEC3B ELISA Kit (Human) (OKCD09303) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Lectin Domain Family 3, Member B (CLEC3B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Type Lectin Domain Family 3, Member B (CLEC3B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Type Lectin Domain Family 3, Member B (CLEC3B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C-Type Lectin Domain Family 3, Member B (CLEC3B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Type Lectin Domain Family 3, Member B (CLEC3B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Type Lectin Domain Family 3, Member B (CLEC3B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CLEC3B Microplate: 96 Wells (12 x 8 Well strips)
  • CLEC3B Lyophilized Standard: 2
  • 100X CLEC3B HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Plasma.
• Sensitivity: < 0.06ng/mL

Target Info

• Gene Symbol: CLEC3B
• Gene Full Name: C-type lectin domain family 3 member B
• Alias Symbols: C-type lectin domain family 3 member B;plasminogen kringle 4-binding protein;tetranectin;tetranectin (plasminogen-binding protein);TN;TNA.
• NCBI Gene Id: 7123
• Protein Name: Tetranectin
• Description of Target: Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis.
• Uniprot ID: P05452
• Protein Accession #: NP_003269.2
• Nucleotide Accession #: NM_003278.2