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Catalog No: OKCD04378
Size:96WELLS
Price: $750.00
SKU
OKCD04378
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Datasheets/ManualsPrintable datasheet for CLEC11A ELISA Kit (Human) (OKCD04378)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)89-99%87-103%81-101%96-104%
EDTA Plasma (n=5)91-103%79-94%78-104%97-105%
Heparin Plasma (n=5)78-90%82-101%99-105%88-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Lectin Domain Family 11, Member A (CLEC11A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Type Lectin Domain Family 11, Member A (CLEC11A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Type Lectin Domain Family 11, Member A (CLEC11A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C-Type Lectin Domain Family 11, Member A (CLEC11A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.94-60ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)85-9995
EDTA Plasma (n=5)93-10197
Heparin Plasma (n=5)93-10196
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Type Lectin Domain Family 11, Member A (CLEC11A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Type Lectin Domain Family 11, Member A (CLEC11A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CLEC11A Microplate96 Wells (12 x 8 Well strips)
CLEC11A Lyophilized Standard2
100X CLEC11A HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.39 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCLEC11A
Gene Full NameC-type lectin domain containing 11A
Alias SymbolsCLECSF3;C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 3;C-type lectin domain family 11 member A;C-type lectin superfamily member 3;LSLCL;lymphocyte secreted C-type lectin;lymphocyte secreted long form of C-type lectin;osteolectin;P47;SCGF;stem cell growth factor;stem cell growth factor; lymphocyte secreted C-type lectin.
NCBI Gene Id6320
Protein NameC-type lectin domain family 11 member A
Description of TargetPromotes osteogenesis by stimulating the differentiation of mesenchymal progenitors into mature osteoblasts (PubMed:27976999). Important for repair and maintenance of adult bone (By similarity).
Uniprot IDQ9Y240
Protein Accession #NP_002966.1
Nucleotide Accession #NM_002975.2
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