Catalog No: OKCD04378
Size:96WELLS
Price: $750.00
SKU
OKCD04378
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/Manuals | Printable datasheet for CLEC11A ELISA Kit (Human) (OKCD04378) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Lectin Domain Family 11, Member A (CLEC11A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Type Lectin Domain Family 11, Member A (CLEC11A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Type Lectin Domain Family 11, Member A (CLEC11A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C-Type Lectin Domain Family 11, Member A (CLEC11A) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.94-60ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Type Lectin Domain Family 11, Member A (CLEC11A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Type Lectin Domain Family 11, Member A (CLEC11A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates and other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.39 ng/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | CLEC11A |
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Gene Full Name | C-type lectin domain containing 11A |
Alias Symbols | CLECSF3;C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 3;C-type lectin domain family 11 member A;C-type lectin superfamily member 3;LSLCL;lymphocyte secreted C-type lectin;lymphocyte secreted long form of C-type lectin;osteolectin;P47;SCGF;stem cell growth factor;stem cell growth factor; lymphocyte secreted C-type lectin. |
NCBI Gene Id | 6320 |
Protein Name | C-type lectin domain family 11 member A |
Description of Target | Promotes osteogenesis by stimulating the differentiation of mesenchymal progenitors into mature osteoblasts (PubMed:27976999). Important for repair and maintenance of adult bone (By similarity). |
Uniprot ID | Q9Y240 |
Protein Accession # | NP_002966.1 |
Nucleotide Accession # | NM_002975.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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