Catalog No: OKEH07757
Size:96T
Price: $725.00
SKU
OKEH07757
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology CLDN5 ELISA Kit (Human) (OKEH07757) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLDN5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLDN5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLDN5 captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=3.9% (n = 20)
Mean Inter-assay CV%: <=11.4% (n = 20)
ELISA Kit Component
ComponentAmount
CLDN5 Microplate96 Wells (12 x 8 Well strips)
CLDN5 Lyophilized Standard2
100X Biotinylated CLDN5 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Plays a major role in tight junction-specific obliteration of the intercellular space.
::Subcellular Location: Cell junction Tight junction Cell membrane Multi-pass membrane protein
::Unigene: Hs.505337
SMR: O00501
String: 9606.ENSP00000384554
::KEGG: hsa:7122
MIM: 602101
Pfam: PF00822
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.78ng/mL
SpecificityNatural and recombinant Human Claudin-5
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCLDN5
Gene Full Nameclaudin 5
Alias SymbolsAWAL;BEC1;claudin-5;CPETRL1;TMDVCF;TMVCF;transmembrane protein deleted in VCFS;transmembrane protein deleted in velocardiofacial syndrome.
NCBI Gene Id7122
Protein NameO00501
Description of TargetClaudin-5
Uniprot IDhttps://www.uniprot.org/uniprot/O00501
Protein Accession #NP_001124333.1
Nucleotide Accession #NM_001130861.1
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