CLDN4 ELISA Kit (Rat) (OKCD08766)

Catalog No: OKCD08766

Size:96WELLS

CLDN4 ELISA Kit (Rat) (OKCD08766) Standard Curve

Product Info

• Predicted Species Reactivity: Rat
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 4 (CLDN4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Claudin 4 (CLDN4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 4 (CLDN4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Claudin 4 (CLDN4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Claudin 4 (CLDN4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Claudin 4 (CLDN4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CLDN4 Microplate: 96 Wells (12 x 8 Well strips)
  • CLDN4 Lyophilized Standard: 2
  • 100X CLDN4 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.060ng/mL

Target Info

• Gene Symbol: CLDN4
• Gene Full Name: claudin 4
• Alias Symbols: CPE-R; CPER; CPETR; CPETR1; WBSCR8; hCPE-R; Clostridium Perfringens Enterotoxin Receptor 1; Williams-Beuren Syndrome Chromosomal Region 8 Protein
• NCBI Gene Id: 304407
• Protein Name: claudin-4
• Description of Target: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
• Uniprot ID: Q5XIT8
• Protein Accession #: NP_001012022.1
• Nucleotide Accession #: NM_001012022.1