CLDN2 Antibody (OABB00927)

Catalog No: OABB00927

Size:100UG

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Anti- Claudin 2 antibody, OABB00927, Western blotting
All lanes: Anti Claudin 2 (OABB00927) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Predicted bind size: 25 kDa
Observed bind size: 25 kDa

Anti- Claudin 2 antibody, OABB00927, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Human|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: WB: The detection limit for Claudin 2 is approximately 0.2ng/lane under reducing conditions.
  Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Claudin-2 is a protein that in humans is encoded by the CLDN2 gene. By genomic sequence analysis, this gene is mapped to chromosome Xq22.3-q23. This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. By expression in a human intestinal epithelial cell line, it was determined that the intestine-specific homeodomain proteins CDX1 and CDX2 activated a reporter plasmid driven by the CLDN2 promoter.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: E.coli-derived human Claudin 2 recombinant protein (Position: A38-V230). Human Claudin 2 shares 92% amino acid (aa) sequence identity with mouse Claudin 2.
• Purification: Affinity Purified
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Rat: Human
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Claudin-2(CLDN2) detection. Tested with WB, IHC-P in Human;Rat.

Target Info

• Gene Symbol: CLDN2
• Gene Full Name: claudin 2
• Alias Symbols: claudin-2;OAZON;SP82.
• NCBI Gene Id: 9075
• Protein Name: Claudin-2
• Description of Target: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
• Uniprot ID: P57739
• Molecular Weight: 24549 MW