CLDN15 ELISA Kit (Human) (OKEH08631)

Catalog No: OKEH08631

Size:96T

Human CLDN15 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology CLDN15 ELISA Kit (Human) (OKEH08631) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLDN15 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLDN15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLDN15 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.2% (n = 20)
  Mean Inter-assay CV%: <=9.2% (n = 20)
• ELISA Kit Component:
  • CLDN15 Microplate: 96 Wells (12 x 8 Well strips)
  • CLDN15 Lyophilized Standard: 2
  • 100X Biotinylated CLDN15 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members function as impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN15 forms tight junctions that mediate the paracellular transport of small monovalent cations along a concentration gradient, due to selective permeability for Na(+), Li(+) and K(+) ions, but selects against Cl(-) ions. Plays an important role in paracellular Na(+) transport in the intestine and in Na(+) homeostasis. Required for normal Na(+)-dependent intestinal nutrient uptake.
  Subcellular Location: Cell junction Tight junction Cell membrane Multi-pass membrane protein Tight junctions form continuous circumferential cell-cell contacts at the borders of apical and lateral cell membranes that seal the intercellular space and show up as strand-like structures in electron microscopy.
  Unigene: Hs.38738
  SMR: P56746
  String: 9606.ENSP00000308870
  KEGG: hsa:24146
  MIM: 615778
  Pfam: PF00822
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 23pg/mL
• Specificity: Natural and recombinant Human Claudin-15
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CLDN15
• Gene Full Name: claudin 15
• Alias Symbols: claudin-15.
• NCBI Gene Id: 24146
• Protein Name: P56746
• Description of Target: Claudin-15
• Uniprot ID: https://www.uniprot.org/uniprot/P56746
• Protein Accession #: NP_001172009.1
• Nucleotide Accession #: NM_001185080.1