Clcn3 Antibody (OASE00256)

Catalog No: OASE00256

Size:100ug

Western Blot analysis of Rat brain membrane lysate showing detection of NMDAR1 NMDA receptor protein using Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody, Clone S308-48. Primary Antibody: Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody at 1:1000.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN1/NR1 Monoclonal Antibody, Clone S308-48. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN1/NR1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GluN1/NR1 Antibody. (D) Composite.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone: S258-5
• Isotype: IgG1
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, IHC, ICC, IF
• Additional Information: Background Info: Detects ~90kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results)
  Scientific Background: Clcn3 mediates the exchange of chloride ions against protons, and functions as an antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. It may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could also help neuronal cells to establish short-term memory.
  Certificate of Analysis: 1ug/mL was sufficient for detection of Clcn3 in 20ug of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Synthetic peptide amino acids 98-115 (cytoplasmic N-terminus) of rat Clcn3
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~90kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results).
• Dilution: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1.  www.phosphosite.org/proteinAction.do?id=5088&showAllSites=true
• Description: Clcn3 mediates the exchange of chloride ions against protons, and functions as an antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. It may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could also help neuronal cells to establish short-term memory.

Target Info

• Gene Symbol: ClCN3
• Gene Full Name: chloride channel, voltage-sensitive 3
• Alias Symbols: CIC3, CLC3, CLCN 3, Chloride channel 3, Chloride channel protein 3, Chloride channel, voltage-sensitive 3, Chloride transporter ClC 3, DKFZp564I0463, H(+)/Cl(-) exchange transporter 3, Chloride transporter ClC-3, ClC-3, H(+)/Cl(-) exchange transporter 3
• NCBI Gene Id: 84360
• Uniprot ID: P51792
• Protein Accession #: NP_445815.2