Clcn3 Antibody : HRP (OASE00256-HRP)

Catalog No: OASE00256-HRP

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Monoclonal
• Clone: S258-5
• Isotype: IgG1
• Host: Mouse
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB, IHC, ICC
• Additional Information: Background Info: Detects ~90kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results)
  Scientific Background: Clcn3 mediates the exchange of chloride ions against protons, and functions as an antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. It may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could also help neuronal cells to establish short-term memory.
  Certificate of Analysis: 1ug/mL was sufficient for detection of Clcn3 in 20ug of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Synthetic peptide amino acids 98-115 (cytoplasmic N-terminus) of rat Clcn3
• Purification: Protein G Purified
• Concentration: 0.6-0.7 mg/ml
• Dilution: 1ug/mL (WB), 0.1-1ug/mL (IHC/ICC), 1-10ug/mL (IF)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1.  www.phosphosite.org/proteinAction.do?id=5088&showAllSites=true

Target Info

• Gene Symbol: Clcn3
• Gene Full Name: chloride channel, voltage-sensitive 3
• Alias Symbols: ClC-3
• NCBI Gene Id: 84360
• Uniprot ID: P51792
• Protein Accession #: NP_445815.2