Catalog No: OKEH04677
Size:96 Wells
Price: $550.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology CLCF1 ELISA Kit (Human) (OKEH04677) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLCF1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for CLCF1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLCF1 captured in well.
ELISA Kit Range0.156 - 10ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 3.5%
Mean Inter-Assay CV: < 7.8%
ELISA Kit Component
Anti-CLCF1 Microplate96 Wells (12 x 8 Well Strips)
CLCF1 Lyophilized Standard2
Sample Diluent1 x 20 mL
CLCF1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Cardiotrophin-like cytokine factor 1

Genome-wide Analyses of Chromatin State in Human Mast Cells Reveal Molecular Drivers and Mediators of Allergic and Inflammatory Diseases. Immunity. 51, 949-965.e6 (2019). 31653482

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCLCF1
Gene Full NameCardiotrophin-like cytokine factor 1
Alias SymbolsCLC, NR6, BSF3, NNT1, BSF-3, CISS2, NNT-1
NCBI Gene Id23529
Protein NameCardiotrophin-like cytokine factor 1
Description of TargetThis gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Uniprot IDQ9UBD9
Protein Accession #NP_001159684.1
Nucleotide Accession #NM_001166212.1
Protein Size (# AA)225
Molecular Weight25 KDa
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