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Catalog No: OKEH04677
Price: $550.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology CLCF1 ELISA Kit (Human) (OKEH04677) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLCF1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for CLCF1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLCF1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 3.5%
Mean Inter-Assay CV: < 7.8%
ELISA Kit Component
Anti-CLCF1 Microplate96 Wells (12 x 8 Well Strips)
CLCF1 Lyophilized Standard2
Sample Diluent1 x 20 mL
CLCF1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Cardiotrophin-like cytokine factor 1

Genome-wide Analyses of Chromatin State in Human Mast Cells Reveal Molecular Drivers and Mediators of Allergic and Inflammatory Diseases. Immunity. 51, 949-965.e6 (2019). 31653482

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCLCF1
Gene Full Namecardiotrophin like cytokine factor 1
Alias SymbolsB-cell stimulatory factor 3;B-cell-stimulating factor 3;BSF3;BSF-3;cardiotrophin-like cytokine factor 1;CISS2;CLC;CRLF1 associated cytokine-like factor 1;neurotrophin-1/B-cell stimulating factor-3;NNT1;NNT-1;novel neurotrophin-1;NR6.
NCBI Gene Id23529
Protein NameQ9UBD9
Description of TargetCardiotrophin-like cytokine factor 1
Uniprot ID
Protein Accession #NP_001159684.1
Nucleotide Accession #NM_001166212.1
Protein Size (# AA)225
Molecular Weight25 KDa
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