CLCF1 ELISA Kit (Human) (OKEH04677)

Catalog No: OKEH04677

Size:96 Wells

Human CLCF1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology CLCF1 ELISA Kit (Human) (OKEH04677) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLCF1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for CLCF1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLCF1 captured in well.
• ELISA Kit Range: 0.156 - 10ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 3.5%
  Mean Inter-Assay CV: < 7.8%
• ELISA Kit Component:
  • Anti-CLCF1 Microplate: 96 Wells (12 x 8 Well Strips)
  • CLCF1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • CLCF1 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.1 ng/mL
• Specificity: Natural and recombinant Human Cardiotrophin-like cytokine factor 1
• Publications:

  Genome-wide Analyses of Chromatin State in Human Mast Cells Reveal Molecular Drivers and Mediators of Allergic and Inflammatory Diseases. Immunity. 51, 949-965.e6 (2019). 31653482

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CLCF1
• Gene Full Name: Cardiotrophin-like cytokine factor 1
• Alias Symbols: CLC, NR6, BSF3, NNT1, BSF-3, CISS2, NNT-1
• NCBI Gene Id: 23529
• Protein Name: Cardiotrophin-like cytokine factor 1
• Description of Target: This gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Uniprot ID: Q9UBD9
• Protein Accession #: NP_001159684.1
• Nucleotide Accession #: NM_001166212.1
• Protein Size (# AA): 225
• Molecular Weight: 25 KDa