CLCF1 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03738)

Catalog No: OKCD03738

Size:96WELLS

Mouse CLCF1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	91-101%	94-105%	86-94%	93-101%
  EDTA Plasma (n=5)	83-97%	86-93%	92-99%	82-96%
  Heparin Plasma (n=5)	79-92%	84-93%	81-101%	79-101%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Cardiotrophin Like Cytokine Factor 1 (CLCF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cardiotrophin Like Cytokine Factor 1 (CLCF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cardiotrophin Like Cytokine Factor 1 (CLCF1) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	87-103	92
  EDTA Plasma (n=5)	99-105	102
  Heparin Plasma (n=5)	80-96	86

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cardiotrophin Like Cytokine Factor 1 (CLCF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cardiotrophin Like Cytokine Factor 1 (CLCF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Clcf1 Microplate: 96 Wells (12 x 8 Well strips)
  • Clcf1 Lyophilized Standard: 2
  • 100X Clcf1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 31.2 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Clcf1
• Gene Full Name: cardiotrophin-like cytokine factor 1
• Alias Symbols: B-cell stimulating factor 3;B-cell-stimulating factor 3;Bsf;Bsf3;BSF-3;cardiotrophin-like cytokine factor 1;CL;CLC;neurotrophin-1/B-cell stimulating factor-3;NNT-1;NNT-1/BS;novel neurotrophin-1.
• NCBI Gene Id: 56708
• Protein Name: Cardiotrophin-like cytokine factor 1
• Description of Target: Cytokine with B-cell stimulating capability. Binds to and activates the ILST/gp130 receptor.
• Uniprot ID: Q9QZM3
• Protein Accession #: NP_001296967
• Nucleotide Accession #: NM_001310038