CLCF1 Antibody (OAGA04548)

Catalog No: OAGA04548

Size:100 ul

NNT-1/BSF-3 antibody detects CLCF1 protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug whole cell lysate/extract of human CLCF1-transfected 293T cells
12 % SDS-PAGE
NNT-1/BSF-3 antibody dilution: 1:5000

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NNT-1/BSF-3 antibody at 1:250 dilution.

Immunofluorescence analysis of methanol-fixed A431, using NNT1 antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NNT-1/BSF-3. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: CLCF1
• Gene Full Name: cardiotrophin like cytokine factor 1
• Alias Symbols: CLC, NR6, BSF3, NNT1, BSF-3, CISS2, NNT-1
• NCBI Gene Id: 23529
• Protein Name: cardiotrophin-like cytokine factor 1
• Description of Target: This gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Uniprot ID: Q9UBD9
• Protein Accession #: NP_001159684.1
• Nucleotide Accession #: NM_001166212.1