CLC ELISA Kit (Human) (OKCD08105)

Catalog No: OKCD08105

Size:96WELLS

CLC ELISA Kit (Human) (OKCD08105) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Charcot Leyden Crystal Protein (CLC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Charcot Leyden Crystal Protein (CLC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Charcot Leyden Crystal Protein (CLC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Charcot Leyden Crystal Protein (CLC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Charcot Leyden Crystal Protein (CLC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Charcot Leyden Crystal Protein (CLC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CLC Microplate: 96 Wells (12 x 8 Well strips)
  • CLC Lyophilized Standard: 2
  • 100X CLC HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.116ng/mL

Target Info

• Gene Symbol: CLC
• Gene Full Name: Charcot-Leyden crystal galectin
• Alias Symbols: Charcot-Leyden crystal protein;eosinophil lysophospholipase;Gal-10;GAL10;galectin-10;lectin, galactoside-binding, soluble, 10;LGALS10;LGALS10A;LPPL_HUMAN;lysolecithin acylhydrolase.
• NCBI Gene Id: 1178
• Protein Name: Galectin-10
• Description of Target: Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg).
• Uniprot ID: Q05315
• Protein Accession #: NP_001819.2
• Nucleotide Accession #: NM_001828.5