CL43 ELISA Kit (Bovine) (OKCD01594)

Catalog No: OKCD01594 (Formerly GWB-KBBZU8)

Size:96WELLS

Bovine CL43 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	92-103%	96-104%	88-102%	88-96%
  EDTA Plasma (n=5)	82-95%	90-98%	80-101%	90-104%
  Heparin Plasma (n=5)	81-96%	94-105%	79-103%	95-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collectin Of 43kDa (CL43). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collectin Of 43kDa (CL43). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collectin Of 43kDa (CL43), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Collectin Of 43kDa (CL43) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	82-93	86
  EDTA Plasma (n=5)	88-98	94
  Heparin Plasma (n=5)	81-101	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collectin Of 43kDa (CL43) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collectin Of 43kDa (CL43) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CL43 Microplate: 96 Wells (12 x 8 Well Strips)
  • CL43 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated CL43 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 0.067 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Collectin Of 43kDa (CL43). No significant cross-reactivity or interference between Collectin Of 43kDa (CL43) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CL43
• Alias Symbols: CL-43
• NCBI Gene Id: 431784
• Protein Name: Collectin-43
• Description of Target: Lectin that binds to various sugars: mannose = ManNAc > fucose > GlcNAc > glucose = maltose > galactose > lactose > GalNAc. Could play a role in immune defense.
• Uniprot ID: P42916
• Protein Accession #: NP_001002237.1
• Nucleotide Accession #: NM_001002237.1
• Protein Size (# AA): 321